Toxoplasma IgG ELISA Kit

Kufotokozera Kwachidule:

Toxoplasma IgG ELISA Kit ndi enzyme yolumikizidwa ndi immunosorbent assay pakuzindikira kwamtundu wa ma antibodies a IgG ku Toxoplasma mu seramu yamunthu kapena plasma.Amapangidwa kuti azigwiritsidwa ntchito m'ma laboratories azachipatala kuti azindikire ndikuwongolera odwala omwe ali ndi matenda a Toxoplasma.Nthawi zambiri, kukwera kwa ma antibodies a Toxoplasma IgM kumawonekera koyamba pambuyo pa matenda a Toxoplasma, omwe amatha kuzindikirika masiku 6 pambuyo pa matenda, kenako amawuka pang'onopang'ono ndikukhala kwa milungu ingapo mpaka miyezi, ndipo pamapeto pake amatsika pang'onopang'ono mpaka kuzimiririka.Ma antibodies a IgG amawonetsa matenda am'mbuyomu a Toxoplasma gondii, omwe nthawi zambiri amakhala osatha, koma amathanso kukhala owopsa.Pambuyo poyesa koyamba kwa ma antibodies a IgG, mayeso omwewo amabwerezedwa pambuyo pa masabata a 2-3 ndipo ngati pali kuwonjezeka kwa 4 kapena kuwonjezereka kwa potency ya ma antibodies, kufufuza kwina kumafunika.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Mfundo yofunika

Chidachi chimazindikira toxoplasma lgG antibody (TOX-lgG) mu seramu yamunthu kapena zitsanzo za plasma, timizere ta polystyrene microwell timakutidwa ndi Toxoplasma antigen.Pambuyo powonjezera seramu kapena zitsanzo za plasma kuti zifufuzidwe, ma antibodies ofanana nawo (TOX-lgG-Ab & ena lgM-Ab) omwe amapezeka mu zitsanzo za odwala amamanga ma antigen pa gawo lolimba, ndipo zigawo zina zosamangika zidzachotsedwa ndi kuchapa.Mu sitepe yachiwiri, HRP (horseradish peroxidase) -conjugated anti-human lgG idzachita makamaka ndi ma antibodies a TOX lgG.Pambuyo kutsuka kuchotsa osamangika HRP-conjugate, chromogen mayankho amawonjezedwa mu zitsime.Pamaso pa (TOX Ag) - (TOX-lgG) - (anti-anthu lgG-HRP) immunocomplex, mutatsuka mbale, gawo lapansi la TMB lidawonjezedwa pakukula kwamtundu, ndipo HRP yolumikizidwa ku zovutazo imathandizira kusintha kwa wopanga utoto. kuti mupange zinthu zabuluu, onjezani 50 µ I wa Stop Solution, ndikusintha chikasu.Kukhalapo kwa kuyamwa kwa antibody ya TOX-lgG mu zitsanzo kunatsimikiziridwa ndi owerenga ma microplate.

Zogulitsa Zamalonda

Kutengeka kwakukulu, kukhazikika komanso kukhazikika

Mafotokozedwe a Zamalonda

Mfundo yofunika	Ma enzyme ogwirizana ndi immunosorbent assay
Mtundu	Njira yosalunjika
Satifiketi	NMPA
Chitsanzo	Seramu ya anthu / plasma
Kufotokozera	48T / 96T
Kutentha kosungirako	2-8 ℃
Alumali moyo	Miyezi 12