Rubella virus IgM ELISA Kit

Chidachi chimazindikira kachilombo ka Rubella IgM antibody (RV-IgM) mu seramu yamunthu kapena zitsanzo za plasma, mizere ya polystyrene microwell imakutidwa kale ndi ma antibodies opita ku puloteni ya immunoglobulin M (anti-µ chain).Mutawonjezera koyamba zitsanzo za seramu kapena plasma kuti ziwunikidwe, ma antibodies a IgM omwe ali pachiwonetsero amatha kugwidwa, ndipo zigawo zina zosamangika (kuphatikiza ma antibodies enieni a IgG) zidzachotsedwa pochapa.Mu sitepe yachiwiri, HRP (horseradish peroxidase) -conjugated antigens idzachita makamaka ndi ma RV IgM antibodies.Pambuyo kutsuka kuchotsa chosamangika cha HRP-conjugate, njira za chromogen zimawonjezeredwa m'zitsime.Pamaso pa (anti-µ) -(RV-lgM) -(RV Ag-HRP) immunocomplex, mutatsuka mbale, gawo laling'ono la TMB linawonjezedwa kuti likulitse mtundu, ndipo HRP yolumikizidwa ku zovutayi imayambitsa zomwe wopanga mtundu amachitira. pangani zinthu zabuluu, onjezani 50 µI ya Stop Solution, ndikusintha chikasu.Kukhalapo kwa kuyamwa kwa antibody ya RV-IgM pachitsanzocho kunatsimikiziridwa ndi owerenga ma microplate.