Human Cytomegalovirus IgM ELISA Kit

Kufotokozera Kwachidule:

Human Cytomegalovirus IgM (HCMV-IgM) ELISA Kit ndi enzyme yolumikizidwa ndi immunosorbent assay yowunikira ma antibodies amtundu wa IgM ku Human Cytomegalovirus mu seramu yamunthu kapena plasma.Amapangidwa kuti azigwiritsidwa ntchito m'ma laboratories azachipatala kuti azindikire ndikuwongolera odwala omwe ali ndi kachilombo ka Human Cytomegalovirus.HCMV ndi chimodzi mwazinthu zofunika kwambiri zomwe zimayambitsa matenda obadwa nawo mwa anthu.Matenda oyambirira kapena atsopano omwe amapezeka mwa amayi apakati amatha kuyambitsa matenda a intrauterine kapena perinatal mwa ana obadwa kumene.80% ya ana omwe ali ndi kachilombo ka HCMV panthawi yomwe ali ndi pakati amatha kukhala ndi zizindikiro monga kusokonezeka maganizo, kusawona bwino, ndi kumva.Matenda oyambirira a HCMV ali ndi zotsatira zoopsa komanso zowopsa kwa odwala omwe ali ndi chitetezo chokwanira, ndipo chiwerengero cha imfa za chibayo cha interstitial chifukwa cha matenda a HCMV m'mafupa a mafupa ndi okwera kwambiri mpaka 75%.Posachedwapa, HCMV yapezeka kuti ndi imodzi mwamagwero a matenda otengera mwayi kwa odwala AIDS.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Mfundo yofunika

Chidachi chimazindikira ma antibody a Human Cytomegalovirus IgM (HCMV-IgM) mu seramu yamunthu kapena zitsanzo za plasma, mizere ya polystyrene microwell imakutidwa kale ndi ma antibodies opita ku protein ya immunoglobulin M (anti-µ chain).Mutawonjezera koyamba zitsanzo za seramu kapena plasma kuti ziwunikidwe, ma antibodies a IgM omwe ali pachiwonetsero amatha kugwidwa, ndipo zigawo zina zosamangika (kuphatikiza ma antibodies enieni a IgG) zidzachotsedwa pochapa.Mu sitepe yachiwiri, HRP (horseradish peroxidase) -conjugated antigens adzakhala makamaka amachita ndi HCMV IgM asilikali.Pambuyo kutsuka kuchotsa chosamangika cha HRP-conjugate, njira za chromogen zimawonjezeredwa m'zitsime.Pamaso pa (anti-µ) -(HCMV-lgM) -(HCMV Ag-HRP) immunocomplex, mutatsuka mbale, gawo laling'ono la TMB lidawonjezedwa kuti likulitse mtundu, ndipo HRP yolumikizidwa ndi zovutazo imathandizira kusintha kwa wopanga utoto. pangani zinthu zabuluu, onjezani 50 µI ya Stop Solution, ndikusintha chikasu.Kukhalapo kwa kuyamwa kwa antibody ya HCMV-IgM pachitsanzocho kudatsimikiziridwa ndi owerenga ma microplate.

Zogulitsa Zamalonda

Kutengeka kwakukulu, kukhazikika komanso kukhazikika

Mafotokozedwe a Zamalonda

Mfundo yofunika	Ma enzyme ogwirizana ndi immunosorbent assay
Mtundu	Jambulani njira
Satifiketi	NMPA
Chitsanzo	Seramu ya anthu / plasma
Kufotokozera	48T / 96T
Kutentha kosungirako	2-8 ℃
Alumali moyo	Miyezi 12