Herpes Simplex virus I IgM ELISA Kit

Chidachi chimazindikira kachilombo ka Herpes Simplex Virus I lgM antibody (HSV1-lgM) mu seramu yamunthu kapena zitsanzo za plasma, mizere ya polystyrene microwell imakutidwa kale ndi ma antibodies opita ku mapuloteni aumunthu a immunoglobulin M (anti-µ chain).Pambuyo powonjezerapo zoyamba za seramu kapena plasma kuti zifufuzidwe, ma antibodies a lgM mu chitsanzo amatha kugwidwa, ndi zigawo zina zosamangika (kuphatikizapo ma antibodies enieni a lgG) zidzachotsedwa ndi kuchapa.Mu sitepe yachiwiri, HRP (horseradish peroxidase) -conjugated antigens idzachita makamaka ndi ma antibodies a HSV1 lgM.Pambuyo kutsuka kuchotsa chosamangika cha HRP-conjugate, njira za chromogen zimawonjezeredwa m'zitsime.Pamaso pa (anti-µ) - (HSV1-lgM) - (HSV1 Ag-HRP) immunocomplex, mutatsuka mbale, gawo laling'ono la TMB linawonjezedwa kuti likhale ndi mtundu, ndipo HRP yolumikizidwa ku zovutayi imapangitsa kuti mapangidwe apangidwe apangidwe. pangani zinthu zabuluu, onjezani 50 µ I wa Stop Solution, ndikusintha chikasu.Kukhalapo kwa kuyamwa kwa antibody ya HSV1-lgM pachitsanzocho kunatsimikiziridwa ndi owerenga ma microplate.