Herpes Simplex II IgM ELISA Kit

Chidachi chimazindikira kachilombo ka Herpes Simplex II IgM antibody (HSV2-IgM) mu seramu yamunthu kapena zitsanzo za plasma, mizere ya polystyrene microwell imakutidwa kale ndi ma antibodies opita ku protein ya immunoglobulin M (anti-µ chain).Mutawonjezera koyamba zitsanzo za seramu kapena plasma kuti ziwunikidwe, ma antibodies a IgM omwe ali pachiwonetsero amatha kugwidwa, ndipo zigawo zina zosamangika (kuphatikiza ma antibodies enieni a IgG) zidzachotsedwa pochapa.Mu sitepe yachiwiri, HRP (horseradish peroxidase) -conjugated antigens idzachita makamaka ndi ma antibodies a HSV2 IgM.Pambuyo kutsuka kuchotsa chosamangika cha HRP-conjugate, njira za chromogen zimawonjezeredwa m'zitsime.Pamaso pa (anti-µ) - (HSV2-lgM) - (HSV2 Ag-HRP) immunocomplex, mutatsuka mbale, gawo laling'ono la TMB linawonjezedwa kuti likulitse mtundu, ndipo HRP yolumikizidwa ku zovutayi imayambitsa kusintha kwa mtundu pangani zinthu zabuluu, onjezani 50 µ I wa Stop Solution, ndikusintha chikasu.Kukhalapo kwa kuyamwa kwa ma antibody a HSV2-IgM pachitsanzocho kudatsimikiziridwa ndi owerenga ma microplate.