Vuto la Hepatitis D IgM ELISA Kit

Chidachi chimazindikira kachilombo ka hepatitis D IgM antibody (HDV-IgM) mu seramu yamunthu kapena zitsanzo za plasma, mizere ya polystyrene microwell imakutidwa kale ndi ma antibodies opita ku mapuloteni a immunoglobulin M aumunthu (anti-μ chain).Mutawonjezera koyamba zitsanzo za seramu kapena plasma kuti ziwunikidwe, ma antibodies a IgM omwe ali pachiwonetsero amatha kugwidwa, ndipo zigawo zina zosamangika (kuphatikiza ma antibodies enieni a IgG) zidzachotsedwa pochapa.Mu sitepe yachiwiri, HRP (horseradish peroxidase) -conjugated antigens idzachita makamaka ndi ma antibodies a HDV IgM.Pambuyo kutsuka kuchotsa chosamangika cha HRP-conjugate, njira za chromogen zimawonjezeredwa m'zitsime.Pamaso pa (anti-μ) - (HDV-IgM) - (HDV Ag-HRP) immunocomplex, mutatsuka mbale, gawo lapansi la TMB linawonjezedwa kuti likhale ndi chitukuko cha mtundu, ndipo HRP yolumikizidwa ku zovutazo imapangitsa kuti mapangidwe apangidwe apangidwe. pangani buluu, onjezerani 50μl ya Stop Solution, ndikusintha chikasu.Kukhalapo kwa kuyamwa kwa antibody ya HDV-IgM pachitsanzocho kunatsimikiziridwa ndi owerenga ma microplate.