Enterovirus 71(EV71) IgM ELISA Kit

Kufotokozera Kwachidule:

Enterovirus 71 IgM (EV71-IgM) ELISA kit ndi enzyme-yolumikizidwa ndi immunosorbent assay yowunikira ma antibodies a IgM-class ku Enterovirus 71 mu seramu yamunthu kapena plasma.Amapangidwa kuti azigwiritsidwa ntchito m'ma laboratories azachipatala kuti azindikire ndikuwongolera odwala omwe ali ndi matenda a Enterovirus 71.

The enterovirus 71 (EV71), membala watsopano kwambiri wa Enteroviridae, ndi chifukwa chofala cha matenda a manja, phazi ndi pakamwa (HFMD) ndipo nthawi zina amagwirizanitsidwa ndi matenda aakulu a mitsempha yapakati.Matendawa nthawi zambiri amakhala pachimake mu June ndi July.Matenda a EV71 amatha kukhala opanda zizindikiro kapena angayambitse kutsekula m'mimba komanso totupa.Ana ochepera zaka zisanu ali pachiwopsezo chachikulu chotenga matenda oopsa kuposa akuluakulu ndi ana okulirapo.

EV71 imapatsirana kuchokera kwa munthu kupita kwa munthu mwa kukhudza mwachindunji komanso kudzera m'manja kapena zinthu zomwe zili ndi ndowe zomwe zili ndi kachilomboka.Kutuluka kwa mphuno ndi mmero, malovu kapena madzi otuluka m'matuza amathanso kufalitsa kachilomboka.

EV71 imatha kudzipatula ndikuzindikirika pakhosi ndi ndowe pogwiritsa ntchito kuyezetsa kwa PCR.Viral RNA wapezeka pakhungu vesicle madzimadzi, magazi ndi mkodzo.Kuzindikira koyambirira komanso kosavuta kutha kupezedwa pogwiritsa ntchito mayeso a serological a EV71, kuphatikiza kuyesa kwa ma antibody a IgM.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Mfundo yofunika

Chidachi chimazindikira antibody ya Enterovirus 71 IgM (EV71-IgM) mu seramu yamunthu kapena zitsanzo za plasma, mizere ya polystyrene microwell imakutidwa kale ndi ma antibodies opita ku mapuloteni a immunoglobulin M (anti-μ chain) . atafufuzidwa, ma antibodies a IgM omwe ali mu chitsanzocho akhoza kugwidwa, ndipo zigawo zina zosamangika (kuphatikizapo ma antibodies enieni a IgG) zidzachotsedwa mwa kuchapa.Mu sitepe yachiwiri, HRP (horseradish peroxidase) -conjugated antigens idzachita makamaka ndi ma antibodies a EV71 IgM.Pambuyo kutsuka kuchotsa chosamangika cha HRP-conjugate, njira za chromogen zimawonjezeredwa m'zitsime.Pamaso pa (anti-μ) - (EV71-IgM) - (EV71-Ag-HRP) immunocomplex, mutatsuka mbale, gawo laling'ono la TMB lidawonjezedwa kuti likhale ndi mtundu, ndipo HRP yolumikizidwa ndi zovutazo imayambitsa zomwe wopanga mtundu. kuti mupange buluu, onjezani 50μL ya Stop Solution, ndikusintha chikasu.Kukhalapo kwa kuyamwa kwa EV71-IgM antibody mu chitsanzocho kunatsimikiziridwa ndi wowerenga microplate.

Zogulitsa Zamalonda

Kutengeka kwakukulu, kukhazikika komanso kukhazikika

Mafotokozedwe a Zamalonda

Mfundo yofunika	Ma enzyme ogwirizana ndi immunosorbent assay
Mtundu	Jambulani njira
Satifiketi	CE
Chitsanzo	Seramu ya anthu / plasma
Kufotokozera	48T / 96T
Kutentha kosungirako	2-8 ℃
Alumali moyo	Miyezi 12