Enterovirus 71(EV71) IgG ELISA Kit
Mfundo yofunika
Chidachi chimazindikira antibody ya Enterovirus 71 IgG (EV71-IgG) mu seramu yamunthu kapena zitsanzo za plasma, mizere ya polystyrene microwell imakutidwa kale ndi antigen ya Enterovirus 71.Pambuyo powonjezerapo zitsanzo za seramu kapena plasma kuti zifufuzidwe, ma antibodies ogwirizana (EV71-IgG-Ab & ena a IgM-Ab) omwe amapezeka mu zitsanzo za odwala amamanga ma antigen pa gawo lolimba, ndipo zigawo zina zosamangika zidzachotsedwa ndi kuchapa.Mu sitepe yachiwiri, HRP (horseradish peroxidase) -conjugated anti-anthu IgG idzachita makamaka ndi ma antibodies a EV71 IgG.Pambuyo kutsuka kuchotsa chosamangika cha HRP-conjugate, njira za chromogen zimawonjezeredwa m'zitsime.Pamaso pa (EV71 Ag) - (EV71-IgG) - (anti-anthu IgG-HRP) immunocomplex, mutatha kutsuka mbale, gawo lapansi la TMB lidawonjezedwa kuti likhale ndi mtundu, ndipo HRP yolumikizidwa ku zovutazo imayambitsa zomwe wopanga mtundu. kuti mupange buluu, onjezani 50μL ya Stop Solution, ndikusintha chikasu.Kukhalapo kwa kuyamwa kwa antibody ya EV71-IgG pachitsanzocho kudatsimikiziridwa ndi wowerenga ma microplate.
Zogulitsa Zamalonda
Kutengeka kwakukulu, kukhazikika komanso kukhazikika
Mafotokozedwe a Zamalonda
Mfundo yofunika | Ma enzyme ogwirizana ndi immunosorbent assay |
Mtundu | Njira yosalunjika |
Satifiketi | CE |
Chitsanzo | Seramu ya anthu / plasma |
Kufotokozera | 48T / 96T |
Kutentha kosungirako | 2-8 ℃ |
Alumali moyo | Miyezi 12 |
Kuyitanitsa Zambiri
Dzina la malonda | Paketi | Chitsanzo |
Enterovirus 71(EV71) IgG ELISA Kit | 48T / 96T | Seramu ya anthu / plasma |