SARS-COV-2 Total Ab Test Kit (Elisa)

SARS-CoV-2 Total Ab Test Kit (ELISA) idakhazikitsidwa pa immunoenzymatic assay kuti adziwe ma antibodies a SARS-CoV-2 mu seramu yamunthu, plasma (EDTA, Heparin kapena sodium citrate).Zitsime zazing'onoting'ono ngati gawo lolimba zimakutidwa ndi mapuloteni a SARS-CoV-2 recombinant Receptor Binding Domain.Mu gawo loyamba la makulitsidwe lolingana ndi ma antibodies enieni (SARS-CoV-2-IgG-Ab & ena a IgM-Ab) omwe amapezeka m'zitsanzo za odwala amamanga ma antigen pagawo lolimba.Kumapeto kwa makulitsidwe unbound zigawo zikuluzikulu zasambitsidwa.Pa gawo lachiwiri la makulitsidwe a SARS-CoV-2 recombinant Receptor Binding Domain protein conjugate (SARS-CoV-2 recombinant Receptor Binding Domain protein peroxidase conjugate) imawonjezedwa yomwe imamangiriza makamaka ku ma antibodies a SARS-CoV-2 (kuphatikiza IgG ndi IgM) zomwe zimapangitsa mapangidwe ofanana immunocomplexes.Pambuyo pa sitepe yachiwiri yotsuka kuti muchotse conjugate yowonjezera, TMB/Substrate imawonjezedwa (step3).Mtundu wa buluu umasintha kukhala wachikasu ukayimitsa kuyankha ndi kuyimitsa.Kuyamwa kwa ma calibrators ndi chitsanzo kumatsimikiziridwa pogwiritsa ntchito ELISA micro plate reader.Zotsatira za zitsanzo za odwala zimapezedwa poyerekezera ndi mtengo wodulidwa.