Kuzindikira kwa Serological kwa Matenda a Manja, Mapazi, ndi Pakamwa

Manja, Mapazi, ndi Matenda a Pakamwa (HFMD) mwachidule

Matenda a Manja, Mapazi, ndi Pakamwa ndi ofala kwambiri mwa ana aang’ono. Imapatsirana kwambiri, imakhala ndi gawo lalikulu la matenda asymptomatic, njira zovuta zopatsirana, komanso kufalikira mwachangu, zomwe zitha kuyambitsa kufalikira kwakanthawi kochepa, zomwe zimapangitsa kuti miliri ikhale yovuta. Panthawi ya miliri, matenda ophatikizika m'masukulu a kindergartens ndi malo osamalira ana, komanso kuphatikizika kwamabanja amilandu, kumatha kuchitika. Mu 2008, HFMD idaphatikizidwa ndi Unduna wa Zaumoyo pakuwongolera matenda opatsirana a Gulu C.

Coxsackievirus A16 (CA16) ndi Enterovirus 71 (EV71) ndi ma virus omwe amayambitsa HFMD. Deta ya Epidemiological imasonyeza kuti CA16 nthawi zambiri imazungulira nthawi imodzi ndi EV71, zomwe zimayambitsa kuphulika kwa HFMD kawirikawiri. Panthawi ya miliri imeneyi, chiwerengero cha matenda a CA16 chimaposa kwambiri cha EV71, chomwe nthawi zambiri chimakhala choposa 60% ya matenda onse. HFMD yoyambitsidwa ndi EV71 ingayambitse kuwonongeka kwa dongosolo lapakati. Kuchuluka kwa milandu yoopsa komanso kufa kwa odwala omwe ali ndi kachilombo ka EV71 ndikokwera kwambiri kuposa omwe ali ndi matenda a enterovirus ena, omwe amafa kwambiri mpaka 10% -25%. Komabe, matenda a CA16 nthawi zambiri samayambitsa matenda osiyanasiyana okhudzana ndi dongosolo lamanjenje monga aseptic meningitis, brainstem encephalitis, ndi poliomyelitis ngati ziwalo. Chifukwa chake, kuzindikira koyambirira koyambirira ndikofunikira kwambiri pakupulumutsa miyoyo ya odwala omwe ali ndi vuto lalikulu.

Kuyezetsa Zachipatala

Kuyesa kwakanthawi kwachipatala kwa HFMD makamaka kumaphatikizapo kuzindikira kwa nucleic acid ya tizilombo toyambitsa matenda komanso kuzindikira kwa antibody serological. Kampani ya Beier imagwiritsa ntchito ma enzyme-linked immunosorbent assay (ELISA) ndi njira zagolide za colloidal kuti apange Enterovirus 71 Antibody Test Kits ndi Coxsackievirus A16 IgM Antibody Test Kits kuti azindikire kusiyana kwa tizilombo toyambitsa matenda a HFMD. Kuzindikira kwa antibody ku Serum kumapereka chidwi kwambiri, kutsimikizika kwabwino, ndipo ndikosavuta, mwachangu, komanso koyenera kuyezetsa zachipatala m'mabungwe azachipatala pamilingo yonse komanso pamaphunziro akulu akulu a miliri.

Zizindikiro Zodziwika Zachindunji ndi Kufunika Kwachipatala kwa EV71 Infection

Kuzindikiritsa kwa matenda a EV71 kumadalira kuzindikira kwa ma antibodies a EV71-RNA, EV71-IgM, ndi EV71-IgG mu seramu, kapena kuzindikira kwa EV71-RNA mu zitsanzo za swab.

Kutsatira matenda a EV71, ma antibodies a IgM amawonekera koyamba, akukwera kwambiri sabata yachiwiri. Ma antibodies a IgG amayamba kuonekera sabata yachiwiri atatenga kachilomboka ndipo amapitilirabe kwa nthawi yayitali. EV71-IgM ndi chizindikiro chofunikira cha matenda oyamba kapena aposachedwa, kuthandizira kuzindikira msanga komanso kuchiza matenda a EV71. EV71-IgG ndichizindikiro chofunikira pakuzindikiritsa kusiyana kwa matenda, chothandiza pakufufuza za matenda ndi kuwunika momwe katemera alili. Kuzindikira kusintha kwa titer ya antibody pakati pa zitsanzo za seramu yapawiri komanso yotsitsimula kumatha kudziwanso momwe matenda a EV71 alili; mwachitsanzo, kuchuluka kwa geometric mowirikiza kanayi kapena kukulirapo kwa antibody titer mu seramu yotsitsimula poyerekeza ndi seramu yowopsa kumatha kuonedwa ngati matenda apano a EV71.

Zizindikiro Zodziwika Zachindunji ndi Kufunika Kwachipatala kwa CA16 Infection

Kuzindikira kwachindunji kwa matenda a CA16 kumadalira kuzindikira kwa ma antibodies a CA16-RNA, CA16-IgM, ndi CA16-IgG mu seramu, kapena kuzindikira kwa CA16-RNA mu zitsanzo za swab.

Kutsatira matenda a CA16, ma antibodies a IgM amawonekera koyamba, akukwera kwambiri sabata yachiwiri. Ma antibodies a IgG amayamba kuonekera sabata yachiwiri atatenga kachilomboka ndipo amapitilirabe kwa nthawi yayitali. CA16-IgM ndi chizindikiro chofunikira cha matenda oyamba kapena aposachedwa.

Kufunika kwa Mayeso Ophatikiza EV71 ndi CA16 Antibody

HFMD imayambitsidwa ndi ma enterovirus angapo, okhala ndi serotypes wamba kukhala EV71 ndi CA16. Kafukufuku akuwonetsa kuti HFMD yoyambitsidwa ndi kachilombo ka CA16 nthawi zambiri imakhala ndi zizindikiro zapamwamba, imakhala ndi zovuta zochepa, komanso kuneneratu zabwino. Mosiyana ndi zimenezi, HFMD yomwe imayambitsidwa ndi EV71 nthawi zambiri imakhala ndi zizindikiro zoopsa kwambiri zachipatala, imakhala ndi chiwerengero chapamwamba cha milandu komanso imfa ya milandu, ndipo nthawi zambiri imagwirizanitsidwa ndi zovuta zapakati pa mitsempha. Zizindikiro zachipatala za HFMD zimakhala zovuta ndipo nthawi zambiri zimakhala zopanda mawonekedwe, zomwe zimapangitsa kuti matenda a chipatala akhale ovuta kwambiri, makamaka kumayambiriro koyambirira. Kufunika kophatikiza kuyezetsa ma antibody a seramu kwagona m'malo mwa njira zodzipatula zomwe zimawononga nthawi komanso zovuta zachikhalidwe, kuzindikira tizilombo toyambitsa matenda motengera serologically, ndikupereka maziko ozindikira matenda, njira zamankhwala, komanso momwe matendawo amakhalira.

Kusanthula Magwiridwe Azinthu

EV71-IgM ELISAZidaKusanthula Kachitidwe

Zotsatira zikuwonetsa:Beier EV71-IgM Test Kit imawonetsa kukhudzika kwakukulu komanso kutsimikizika kwabwino pakuyesa seramu kuchokera kwa anthu omwe ali ndi kachilombo ka EV71. Gwero la data: National Institute for Viral Disease Control and Prevention, Chinese CDC.

EV71-IgG ELISA Kit Performance Analysis (I)

EV71-IgG ELISA Kit Performance Analysis (II)

Zotsatira zikuwonetsa:Beier EV71-IgG Test Kit ikuwonetsa kuchuluka kwa seramu kuchokera kwa anthu omwe ali ndi matenda a EV71 obwereza. Gwero la data: National Institute for Viral Disease Control and Prevention, Chinese CDC.

CA16-IgM ELISA Kit Performance Analysis

Zotsatira zikuwonetsa:Beier CA16-IgM Test Kit imawonetsa kuzindikirika kwapamwamba komanso kugwirizana kwabwino. Gwero la data: National Institute for Viral Disease Control and Prevention, Chinese CDC.

EV71-IgM Test Kit (Colloidal Gold) Kusanthula Kachitidwe

Zotsatira zikuwonetsa:Beier EV71-IgM Test Kit (Colloidal Gold) imawonetsa kukhudzika kwakukulu komanso kutsimikizika kwabwino pakuyesa seramu kuchokera kwa anthu omwe ali ndi kachilombo ka EV71. Gwero la data: National Institute for Viral Disease Control and Prevention, Chinese CDC.

CA16-IgM Test Kit (Colloidal Gold) Kuwunika Ntchito

Zotsatira zikuwonetsa:Beier CA16-IgM Test Kit (Colloidal Gold) imawonetsa kukhudzika kwakukulu komanso kutsimikizika kwabwino pozindikira seramu kuchokera kwa anthu omwe ali ndi kachilombo ka CA16. Gwero la data: National Institute for Viral Disease Control and Prevention, Chinese CDC.