Kuzindikira kwa HFRS - Hemorrhagic Fever yokhala ndi Renal Syndrome

Mbiri

Kachilombo ka Hantaan (HV) ndiye kachilombo koyambitsa matenda a Hemorrhagic Fever yokhala ndi Renal Syndrome (HFRS). HFRS ndi matenda opatsirana padziko lonse lapansi omwe amadziwika ndi kutentha thupi, kutaya magazi, komanso kuwonongeka kwa aimpso. Matendawa amayamba kwambiri, amapita patsogolo mofulumira, komanso amafa kwambiri, zomwe zimawopseza kwambiri thanzi la anthu. Makoswe (monga Apodemus agrarius ndi Rattus norvegicus) ndizomwe zimasungiramo madzi ndi ma vectors a HV. Kupatsirana kwa anthu kumachitika makamaka kudzera m'chimbudzi cha aerosolized (mkodzo, ndowe, malovu), kukhudzana mwachindunji, kapena kulumidwa ndi vector. HFRS ikhoza kuchitika chaka chonse, ndipo anthu ambiri amatha kutenga kachilomboka. Malinga ndi ziwerengero za WHO, mayiko 32 padziko lonse lapansi anena za miliri ya HV, makamaka ku East Asia, Europe, ndi Balkan.

Antibody Markers Post HV Infection

Kutsatira matenda a HV, chitetezo cha mthupi cha munthu chimapanga ma antibodies enieni, makamaka HV-IgM ndi HV-IgG.

● Ma antibodies a HV-IgM: Amagwira ntchito ngati chizindikiro cha serological ya matenda adakali aang'ono, omwe nthawi zambiri amawonekera pasanathe masiku zizindikiro zayamba, ndipo ndi ofunikira kwambiri kuti adziwe matenda a pachimake.

● Ma antibodies a HV-IgG: Amatuluka pambuyo pake ndipo akhoza kukhalabe moyo wonse, kusonyeza matenda apitalo kapena kuchira. Kuwonjezeka kowirikiza kanayi kapena kukulirapo kwa ma antibody a HV-IgG pakati pa zitsanzo za seramu yapamtima ndi yotsitsimula ndikuzindikiritsanso matenda owopsa.

Njira Zodziwika bwino za HV

Njira zamakono zodziwira HV zikuphatikiza kudzipatula kwa ma virus, PCR, serological ELISA, ndi ma colloidal gold immunoassays.

● Chikhalidwe cha ma virus ndi PCR zimapereka mawonekedwe apamwamba koma zimatenga nthawi, zimafuna luso laukadaulo, ndipo zimafuna malo apamwamba a labotale, zomwe zimalepheretsa kugwiritsidwa ntchito kwawo kofala.

● Micro-immunofluorescence (MIF) imapereka kulondola kwabwino koma imafunikira maikulosikopu a fluorescence ndi kutanthauzira kwa akatswiri, kuletsa kugwiritsa ntchito mwachizolowezi.

● ELISA ndi kuyesa kwa golidi wa colloidal amavomerezedwa kwambiri m'machipatala chifukwa cha kuphweka kwawo, kuthamanga, kukhudzidwa kwakukulu ndi tsatanetsatane, komanso kusonkhanitsa zitsanzo mosavuta (seramu / plasma).

Magwiridwe Azinthu

Mayeso a Beier Bio a HV-IgM/IgG (ELISA)

● Mtundu Wachitsanzo: Seramu, plasma

● Dilution Zitsanzo: Mayesero onse a IgM ndi IgG amagwiritsa ntchito zitsanzo zoyambira bwino zokhala ndi dilution ya 1:11 (100µl sample diluent + 10µl sample), kuwongolera kayendetsedwe ka ntchito ndi kuchepetsa kuchuluka kwa ntchito.

● Reagent Yokonzeka Kugwiritsa Ntchito: Ma reagents onse ali okonzeka kupatula kuchapa (20 × concentrated). Zolemba zamitundu kuti zizindikirike mosavuta

● Njira Yoyalitsira: 30 min / 30 min / 15 min; zodziwikiratu kwathunthu

● Kuzindikira Wavelength: 450 nm ndi 630 nm reference

● Zingwe Zokutira: Zitsime 96 kapena 48 zomwe zimatha kusweka, chilichonse chimakhala ndi khodi yosindikizidwa kuti mufufuze komanso kuti musavutike.

Mayeso a Beier Bio a HV-IgM/IgG (Colloidal Gold)

● Mtundu Wachitsanzo: Seramu

● Kuzindikira Nthawi: Zotsatira mkati mwa mphindi 15; palibe zida zowonjezera zofunika; abwino kuwunika mwachangu m'malo odwala omwe ali kunja, mwadzidzidzi, komanso omwazika odwala

● Ndondomeko: Onjezani chitsanzo cha 10µl ku chitsanzo cha khadi loyesera bwino pogwiritsa ntchito dropper; tanthauzirani zotsatira mkati mwa mphindi 15-20

Magwiridwe Achipatala a HV-IgM (ELISA), HV-IgG (ELISA), ndi HV-IgM/IgG (Colloidal Gold)