Mycoplasma Pneumonia IgM ELISA Kit
Mfundo yofunika
Chidachi chimazindikira Mycoplasma Pneumoniae IgM (MP-IgM) mu seramu ya anthu kapena zitsanzo za plasma, mizere ya polystyrene microwell imakutidwa kale ndi ma antibodies opita ku mapuloteni a immunoglobulin M (anti-μ chain). , ma antibodies a IgM mu chitsanzo amatha kugwidwa, ndipo zigawo zina zosamangika (kuphatikizapo ma antibodies enieni a IgG) zidzachotsedwa ndi kuchapa.Mu sitepe yachiwiri, HRP(horseradish peroxidase) -conjugated antigens idzachita makamaka ndi ma antibodies a MP IgM.Pambuyo kutsuka kuchotsa chosamangika cha HRP-conjugate, njira za chromogen zimawonjezeredwa m'zitsime.Pamaso pa (anti-μ) - (MP-IgM) - (MP-Ag-HRP) immunocomplex, mutatsuka mbale, gawo laling'ono la TMB linawonjezedwa kuti likhale ndi chitukuko cha mtundu, ndipo HRP yolumikizidwa ku zovutazo imayambitsa zomwe wopanga mtundu. kuti mupange buluu, onjezani 50μl ya Stop Solution, ndikusintha chikasu.Kukhalapo kwa kuyamwa kwa anti-MP-IgM m'chitsanzocho kunatsimikiziridwa ndi owerenga ma microplate.
Zogulitsa Zamalonda
Kutengeka kwakukulu, kukhazikika komanso kukhazikika
Mafotokozedwe a Zamalonda
Mfundo yofunika | Ma enzyme ogwirizana ndi immunosorbent assay |
Mtundu | Jambulani Njira |
Satifiketi | NMPA |
Chitsanzo | Seramu ya anthu / plasma |
Kufotokozera | 96t ndi |
Kutentha kosungirako | 2-8 ℃ |
Alumali moyo | Miyezi 12 |
Kuyitanitsa Zambiri
Dzina la malonda | Paketi | Chitsanzo |
Mycoplasma Pneumoniae IgM ELISA Kit | 96t ndi | Seramu ya anthu / plasma |