Measles Virus (MV) IgG ELISA Kit

Chidachi chimazindikira kachilombo ka chikuku IgG antibody (MV-IgG) mu seramu yamunthu kapena zitsanzo za plasma, mizere ya polystyrene microwell imakutidwa kale ndi Measles virus antigen.Mutawonjezera koyamba zitsanzo za seramu kapena plasma kuti zifufuzidwe, ma antibodies ofanana (MV-IgG-Ab & ena a IgM-Ab) omwe amapezeka mu zitsanzo za odwala amamanga ma antigen pa gawo lolimba, ndipo zigawo zina zosamangika zidzachotsedwa ndi kuchapa.Mu sitepe yachiwiri, HRP(horseradish peroxidase) -conjugated anti-human IgG idzachitapo kanthu kokha ndi ma MV IgG antibodies.Pambuyo kutsuka kuchotsa chosamangika cha HRP-conjugate, njira za chromogen zimawonjezeredwa m'zitsime.Pamaso pa (MV Ag) - (MV-IgG) - (anti-anthu IgG-HRP) immunocomplex, pambuyo kutsuka mbale, ndi TMB gawo lapansi anawonjezedwa kwa mtundu chitukuko, ndi HRP chikugwirizana ndi zovuta catalyzes mtundu wopanga mapulogalamu anachita. kupanga buluu, kuwonjezera 50μl ya Stop Solution, ndi kutembenukira chikasu.Kupezeka kwa kuyamwa kwa antibody ya MV-IgG mu chitsanzo kunatsimikiziridwa ndi owerenga microplate.