Anti-Zona Pellucida (ZP) Antibody ELISA Kit

Kufotokozera Kwachidule:

Izi zidapangidwa kuti zizizindikirika bwino mu vitro ma antibodies a zona pellucida (ZP) mu seramu yamunthu. Zona pellucida, matrix apadera ozungulira ma oocyte, amagwira ntchito yofunika kwambiri pakuzindikirika kwa umuna, kumanga, ndi umuna, komanso kukula kwa umuna.

Ma antibodies a ZP ndi ma antibodies omwe amayang'ana ma antigen a zona pellucida. Pamene iwo kuonekera mu thupi, iwo akhoza mwachindunji kumanga kuti zona pellucida, kutsekereza yachibadwa kucheza pakati pa umuna ndi oocytes, potero kulepheretsa umuna. Kuphatikiza apo, amatha kusokoneza njira yoyika mazira omwe ali ndi umuna, chomwe ndi chimodzi mwazinthu zofunika kwambiri zomwe zimayambitsa kusabereka kwa autoimmune.

Kachipatala, kuzindikira uku kumagwira ntchito ngati njira yodziwira matenda a autoimmune infertility. Pozindikira kuchuluka kwa ma antibodies a ZP mu seramu ya odwala, imatha kupereka chidziwitso chofunikira chofotokozera zomwe zimayambitsa kusabereka mwa odwala ena omwe ali ndi zifukwa zosadziwika, kuthandiza madokotala kupanga njira zowunikira komanso zamankhwala.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zogulitsa Tags

Mfundo yofunika

Chidachi chimazindikira ma antibodies a zona pellucida (ZP-Ab) mu zitsanzo za seramu yamunthu kutengera njira yosadziwika, ndi zoyeretsedwa zona pellucida zomwe zimagwiritsidwa ntchito ngati antigen.

Njira yoyesera imayamba ndikuwonjezera zitsanzo za seramu ku zitsime zomwe zidakutidwa kale ndi antigen, ndikutsatiridwa ndi makulitsidwe. Ngati ZP-Ab ilipo pachitsanzocho, imamangiriza ku antigen ya zona pellucida m'zitsime, ndikupanga ma antigen-antibody complexes.

Kenako, ma enzyme conjugates amawonjezeredwa ku zitsime. Pambuyo pa gawo lachiwiri la makulitsidwe, ma enzyme awa amalumikizana ndi ma antigen-antibody complexes omwe alipo. Pamene njira ya TMB ya gawo lapansi ikayambitsidwa, mawonekedwe amtundu amapezeka pansi pa mphamvu ya enzyme muzovuta. Pomaliza, chowerengera cha microplate chimagwiritsidwa ntchito kuyeza kuyamwa (mtengo A), zomwe zimalola kutsimikiza kwa milingo ya ZP-Ab mu zitsanzo.

Zamalonda

Kutengeka kwakukulu, kukhazikika komanso kukhazikika

Mafotokozedwe a Zamalonda

Mfundo yofunika	Ma enzyme ogwirizana ndi immunosorbent assay
Mtundu	ZosalunjikaNjira
Satifiketi	NMPA
Chitsanzo	Seramu ya anthu / plasma
Kufotokozera	48T /96T
Kutentha kosungirako	2-8℃
Alumali moyo	12miyezi