Anti-Ovarian (AO) Antibody ELISA Kit

Kufotokozera Kwachidule:

The ovary lili mazira, zona pellucida, granulosa maselo, etc., pa magawo osiyanasiyana chitukuko. Chigawo chilichonse chingapangitse ma anti-ovarian antibodies (AoAb) chifukwa cha ma antigen achilendo. Kuwonongeka kwa ovarian antigen chifukwa cha kuvulala kwa ovarian, matenda, kapena kutupa kungayambitse AoAb mwa anthu omwe ali ndi vuto la chitetezo chamthupi. AoAb imawononganso ovary ndikusokoneza magwiridwe antchito a chiberekero ndi placenta, zomwe zimapangitsa kusabereka komanso kupititsa padera.

AoAb idapezeka koyamba mwa odwala omwe ali ndi vuto la ovarian msanga (POF) komanso amenorrhea yoyambirira, yolumikizidwa ndi machitidwe a autoimmune. AoAb poyamba imachepetsa kubereka ndipo pamapeto pake imayambitsa kulephera kwa ovary. Odwala osabereka omwe ali ndi AoAb zabwino koma palibe POF angakumane ndi zoopsa zamtsogolo za POF, zomwe zimafuna kuunika kwa ovarian reserve.

AoAb positivity ndi yochuluka mwa odwala osabereka komanso opita padera, kusonyeza ubale wapamtima. Kafukufuku akuwonetsa kuti AoAb imayambitsa kusabereka kuposa kupititsa padera. Kafukufuku waposachedwa amazindikira AoAb mwa odwala ambiri a PCOS, kutanthauza kuti kutupa kwa ovary komwe kumapangidwa ndi chitetezo chamthupi komanso ma cytokines osadziwika kungayambitse PCOS ndi kusabereka, zomwe zimafunika kuphunzira mopitilira muyeso.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zogulitsa Tags

Mfundo yofunika

Chidachi chimazindikira ma anti-ovarian antibodies (IgG) m'miyeso ya seramu yamunthu kutengera njira yosadziwika, yokhala ndi ma antigen oyeretsedwa amtundu wa ovarian omwe amagwiritsidwa ntchito pophimba tinthu tating'onoting'ono.

Njira yoyesera imayamba ndikuwonjezera zitsanzo za seramu ku zitsime za antigen-precoated reaction to incubation. Ngati ma anti-ovarian antibodies alipo pachitsanzocho, amamanga makamaka ku ma antigen omwe anali atakutidwa kale mu ma microwells, kupanga ma antigen-antibody complexes okhazikika. Zigawo zosamangika zimachotsedwa kuti zitsimikizire kuti zizindikirika zolondola.

Kenako, ma antibodies a horseradish peroxidase (HRP) -olembedwa ndi mbewa odana ndi anthu a IgG amawonjezeredwa ku zitsimezo. Pambuyo pa kukulitsidwa kwachiwiri, ma antibodies olembedwa ndi enzymewa amamanga mwachindunji ku anti-ovarian antibodies m'magulu omwe alipo a antigen-antibody, kupanga "antigen-antibody-enzyme label" yathunthu ya chitetezo cha mthupi.

Pomaliza, yankho la gawo la TMB lawonjezeredwa. HRP muzovuta imathandizira kusintha kwamankhwala ndi TMB, kutulutsa kusintha kowoneka. The absorbance (A value) ya reaction solution imayesedwa pogwiritsa ntchito microplate reader, ndipo kupezeka kapena kusapezeka kwa anti-ovarian antibodies mu chitsanzocho kumatsimikiziridwa potengera zotsatira za kuyamwa.

Zamalonda

Kutengeka kwakukulu, kukhazikika komanso kukhazikika

Mafotokozedwe a Zamalonda

Mfundo yofunika	Ma enzyme ogwirizana ndi immunosorbent assay
Mtundu	ZosalunjikaNjira
Satifiketi	NMPA
Chitsanzo	Seramu ya anthu / plasma
Kufotokozera	48T /96T
Kutentha kosungirako	2-8℃
Alumali moyo	12miyezi