Anti-Cyclic Citrullinated Peptide (CCP) Antibody ELISA Kit

Chidachi chimazindikira ma anti-cyclic citrullinated peptide antibodies (CCP antibodies) mu zitsanzo za seramu ya anthu kutengera njira yosadziwika, yokhala ndi ma antigen oyeretsedwa a cyclic citrullinated peptide omwe amagwiritsidwa ntchito ngati antigen.

Njira yoyesera imayamba ndikuwonjezera zitsanzo za seramu ku zitsime zomwe zidakutidwa kale ndi ma antigen oyeretsedwa omwe tawatchulawa, ndikutsatiridwa ndi nthawi yoyambira. Pamakulitsidwe awa, ngati ma antibodies a CCP alipo pachitsanzocho, amazindikira ndikumanga ma antigen a cyclic citrullinated peptide omwe amakutidwa pa ma microwells, ndikupanga ma antigen-antibody okhazikika. Kuonetsetsa kulondola kwa masitepe wotsatira, unbound zigawo zikuluzikulu mu anachita zitsime amachotsedwa mwa njira kutsuka, amene amathandiza kuthetsa kuthekera kusokoneza zinthu zina mu seramu.

Kenako, ma enzyme conjugates amawonjezedwa ku zitsime zomwe zimachitika. Pambuyo pa kukulitsidwa kwachiwiri, ma enzyme conjugates amalumikizana mwachindunji ndi ma antigen-antibody complexes omwe alipo, kupanga chitetezo chachikulu chomwe chimaphatikizapo antigen, antibody, ndi enzyme conjugate. Pamene njira ya TMB ya gawo lapansi ikalowetsedwa mu dongosolo, puloteni mu conjugate imapangitsa kuti mankhwala asokonezeke ndi gawo lapansi la TMB, zomwe zimapangitsa kuti mtundu usinthe. Kuchuluka kwa mtundu uwu kumagwirizana mwachindunji ndi kuchuluka kwa ma antibodies a CCP omwe amapezeka mu seramu yoyambirira ya seramu. Pomaliza, owerenga microplate amagwiritsidwa ntchito kuyeza kuyamwa (A mtengo) wa zomwe zimasakanikirana. Pakuwunika kuchuluka kwa kuyamwa uku, kuchuluka kwa ma antibodies a CCP muzachitsanzo kumatha kutsimikiziridwa molondola, kupereka maziko odalirika pakuyezetsa kuchipatala koyenera komanso kuzindikira.